3B9T
Crystal structure of predicted acetamidase/formamidase (YP_546212.1) from Methylobacillus flagellatus KT at 1.58 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.165, 82.723, 83.576 |
| Unit cell angles | 107.82, 105.81, 95.18 |
Refinement procedure
| Resolution | 29.814 - 1.580 |
| R-factor | 0.14 |
| Rwork | 0.138 |
| R-free | 0.17700 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.508 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.814 | 29.814 | 1.620 |
| High resolution limit [Å] | 1.580 | 7.070 | 1.580 |
| Rmerge | 0.090 | 0.074 | 0.459 |
| Total number of observations | 4436 | 27870 | |
| Number of reflections | 196551 | ||
| <I/σ(I)> | 7.2 | 8.4 | 1.6 |
| Completeness [%] | 95.8 | 97.8 | 94.2 |
| Redundancy | 1.9 | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | NANODROP, 1.0M LiCl, 20.0% PEG 6000, 0.1M MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
