3B9K
Crystal structure of CD8alpha-beta in complex with YTS 156.7 FAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 93.2 |
| Detector technology | CCD |
| Collection date | 2005-07-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.779, 92.747, 190.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.330 - 2.700 |
| R-factor | 0.238 |
| Rwork | 0.235 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.576 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.131 | 0.579 |
| Number of reflections | 45395 | |
| <I/σ(I)> | 9.6 | 2.17 |
| Completeness [%] | 97.5 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 6.2 | 296 | PEG 8000, 0.1 M Na/K phosphate, 0.2 M NaCl, pH 6.2, sitting drop, temperature 296K |
