3B8H
Structure of the eEF2-ExoA(E546A)-NAD+ complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 329.440, 68.160, 191.630 |
| Unit cell angles | 90.00, 103.28, 90.00 |
Refinement procedure
| Resolution | 24.992 - 2.500 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25600 |
| Starting model (for MR) | 2b82 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.637 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.138 | 0.683 |
| Number of reflections | 143628 | 15781 |
| <I/σ(I)> | 9.79 | 2.1 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 7% PEG-10000, 3.5mM MPD, 100 mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
