3B8F
Crystal structure of the cytidine deaminase from Bacillus anthracis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.078, 98.479, 61.360 |
Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
Resolution | 57.260 - 1.900 |
R-factor | 0.16908 |
Rwork | 0.168 |
R-free | 0.19589 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.364 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.260 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.092 | 0.683 |
Number of reflections | 45458 | |
<I/σ(I)> | 22.2 | 1.43 |
Completeness [%] | 96.2 | 83.56 |
Redundancy | 4.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M HEPES, 1.4M Tri-sodium citrate dihydrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |