3B7G
Human DEAD-box RNA helicase DDX20, Conserved domain I (DEAD) in complex with AMPPNP (Adenosine-(Beta,gamma)-imidotriphosphate)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00595 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 63.560, 63.560, 214.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.430 - 1.900 |
R-factor | 0.17368 |
Rwork | 0.172 |
R-free | 0.20219 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oxc |
RMSD bond length | 0.014 |
RMSD bond angle | 1.648 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.081 | 0.126 |
Number of reflections | 40642 | |
<I/σ(I)> | 32.1 | 21.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 20.5 | 18.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG 3350, 200mM Ammonium acetate, 100mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |