3B72
Crystal structure of lysozyme folded in SDS and 2-methyl-2,4-pentanediol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-11 |
| Detector | Ruby CCD detector |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.900, 77.900, 37.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.2044 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z55 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.026 |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.500 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.123 | 0.308 |
| Number of reflections | 19000 | |
| <I/σ(I)> | 13.7 | 1.4 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 50mM Tris pH8, 70% MPD and 6mM SDS, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
