3B6Y

Crystal Structure of the Second HIN-200 Domain of Interferon-Inducible Protein 16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-24
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97800
Spacegroup name	P 21 21 21
Unit cell lengths	42.999, 92.887, 100.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.140 - 2.350
R-factor	0.229
Rwork	0.226
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oq0
RMSD bond length	0.016
RMSD bond angle	1.466
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.066	0.048	0.379
Number of reflections	17421
<I/σ(I)>	12.4
Completeness [%]	99.9	99.5	100
Redundancy	7.2	6.6	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	30% PEG 4000, 0.2M Ammonium sulfate, 0.1M Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K