3B6U

Crystal structure of the motor domain of human kinesin family member 3B in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-08-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	58.731, 95.852, 63.883
Unit cell angles	90.00, 92.61, 90.00

Refinement procedure

Resolution	19.860 - 1.800
R-factor	0.192
Rwork	0.191
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1goj
RMSD bond length	0.016
RMSD bond angle	1.458
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.860
High resolution limit [Å]	1.800	3.870	1.800
Rmerge	0.050	0.028	0.377
Number of reflections	64524
<I/σ(I)>	17.1
Completeness [%]	98.9	99.9	96.7
Redundancy	3.6	3.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	291	30% PEG 3350, 0.1 M Tris-HCl, 0.2M Lithium sulfate, pH 8.5, VAPOR DIFFUSION, temperature 291K