3B6L
Crystal structure of lysozyme folded in SDS and 2-methyl-2,4-pentanediol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-23 |
| Detector | BRUKER PROTEUM |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.595, 77.595, 37.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.700 - 2.300 |
| R-factor | 0.20236 |
| Rwork | 0.202 |
| R-free | 0.21400 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 1z55 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.781 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.082 | 0.022 |
| Number of reflections | 5468 | |
| <I/σ(I)> | 21.9 | 8.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 300 | 100mM sodium acetate buffer pH 4.6 and 2M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
