3B6G
Nucleosome core particle treated with oxaliplatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2007-02-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.298, 109.655, 181.807 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.070 - 3.450 |
| R-factor | 0.341 |
| Rwork | 0.339 |
| R-free | 0.43500 |
| Starting model (for MR) | 1kx5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.276 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 94.072 | 109.760 | 3.640 |
| High resolution limit [Å] | 3.450 | 10.910 | 3.450 |
| Rmerge | 0.071 | 0.035 | 0.485 |
| Total number of observations | 3883 | 30745 | |
| Number of reflections | 28350 | ||
| <I/σ(I)> | 6.3 | 18.1 | 1.5 |
| Completeness [%] | 98.9 | 70 | 100 |
| Redundancy | 7.3 | 5.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | MnCl2, KCl, K-Cacodylate, pH 6.0, vapor diffusion, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MnCl2 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | K-Cacodylate | ||
| 4 | 1 | 2 | MnCl2 | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | K-Cacodylate |
