3B61

EmrE multidrug transporter, apo crystal form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0057, 1.0089, 1.0067
Spacegroup name	F 2 2 2
Unit cell lengths	181.000, 239.200, 284.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 4.500
Rwork	0.318
R-free	0.36200
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.660
High resolution limit [Å]	3.000	4.500
Number of reflections	13836
<I/σ(I)>	12.2
Completeness [%]	75.8	38.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	20 mM NaCl, 20 mM sodium acetate, 200-600 mM ammonium sulfate, 15-30% (w/v) PEG-200, and 0.3-0.6% (w/v) NG, pH 4, VAPOR DIFFUSION, SITTING DROP, temperature 298K