3B5W
Crystal Structure of Eschericia coli MsbA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-02-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 107.790, 126.070, 206.560 |
Unit cell angles | 83.47, 76.25, 84.07 |
Refinement procedure
Resolution | 19.980 - 5.300 |
Rwork | 0.276 |
R-free | 0.31100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 5.300 |
Number of reflections | 36078 |
Completeness [%] | 96.8 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop vapor diffusion | 7.5 | 277 | 15-20% PEG 300, 80-120mM LiSO4 and 0.05% alpha-DDM., pH 7.5, sitting drop vapor diffusion, temperature 277K |