3B5T
Crystal Structure of Novel Immune-Type Receptor 10 Se-Met Extracellular Fragment Mutant N30D
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 90.305, 90.305, 137.402 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.750 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.21700 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.057 | 0.383 |
Number of reflections | 66060 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6M Na Citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |