3B5H

Crystal structure of the extracellular portion of HAb18G/CD147

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-29
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	126.481, 126.481, 169.926
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.600 - 2.800
R-factor	0.245
Rwork	0.243
R-free	0.29400
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.460
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.106	0.586
Number of reflections	34141
<I/σ(I)>	11.7
Completeness [%]	99.4	100
Redundancy	7.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.5M ammonium sulfate, 0.1M triSodium Citrate dihydrate pH 5.60, 1.0M Lithium Sulfate monohydrate, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.5M ammonium sulfate, 0.1M triSodium Citrate dihydrate pH 5.60, 1.0M Lithium Sulfate monohydrate, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.5M ammonium sulfate, 0.1M triSodium Citrate dihydrate pH 5.60, 1.0M Lithium Sulfate monohydrate, VAPOR DIFFUSION, HANGING DROP, temperature 277K