3B58
Minimally Junctioned Hairpin Ribozyme Incorporates A38G Mutation and a 2',5'-Phosphodiester Linkage at the Active Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.918 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.370, 93.370, 132.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.800 - 2.650 |
R-factor | 0.237 |
Rwork | 0.236 |
R-free | 0.26200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2p7f |
RMSD bond length | 0.007 |
RMSD bond angle | 1.600 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 2.740 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 10328 | |
<I/σ(I)> | 20.7 | 3.8 |
Completeness [%] | 99.1 | 99.4 |
Redundancy | 10.2 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 2K MME, lithium sulfate, HEPES, spermidine, cobalt hexaammine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 2K MME | ||
2 | 1 | 1 | Li2SO4 | ||
3 | 1 | 1 | HEPES | ||
4 | 1 | 1 | spermidine | ||
5 | 1 | 1 | cobalt hexaammine | ||
6 | 1 | 2 | PEG 2K MME | ||
7 | 1 | 2 | Li2SO4 | ||
8 | 1 | 2 | HEPES | ||
9 | 1 | 2 | spermidine |