3B4U
Crystal structure of dihydrodipicolinate synthase from Agrobacterium tumefaciens str. C58
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97940 |
Spacegroup name | P 1 |
Unit cell lengths | 44.116, 49.442, 69.084 |
Unit cell angles | 79.35, 89.06, 83.94 |
Refinement procedure
Resolution | 67.880 - 1.200 |
R-factor | 0.12846 |
Rwork | 0.127 |
R-free | 0.15559 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.484 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.880 | 1.231 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.077 | 0.274 |
Number of reflections | 157988 | |
<I/σ(I)> | 17.89 | 3 |
Completeness [%] | 93.2 | 72.49 |
Redundancy | 4.7 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Mg formate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |