3B4Q
Crystal structure of a conserved protein domain (unknown function) from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.658, 103.857, 26.329 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.520 - 1.550 |
| R-factor | 0.18621 |
| Rwork | 0.185 |
| R-free | 0.21580 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.581 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.520 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.069 | 0.446 |
| Number of reflections | 25547 | |
| <I/σ(I)> | 38 | 2.4 |
| Completeness [%] | 94.6 | 65.8 |
| Redundancy | 6.4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M Li2SO4, 0.1M Tris-HCl, 30% PEG 3000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
