3B46
Crystal Structure of Bna3p, a Putative Kynurenine Aminotransferase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL1-5 |
Synchrotron site | SSRL |
Beamline | BL1-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97944 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.780, 66.520, 115.200 |
Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
Resolution | 28.800 - 2.000 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w7l |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.065 | 0.255 |
Number of reflections | 60609 | |
<I/σ(I)> | 36.9 | 4.7 |
Completeness [%] | 98.6 | 93.6 |
Redundancy | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 10 mM Tris, 50 mM NaCl, 14 mM mercaptoethanol, 0.025 mM PLP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |