3B40
Crystal structure of the probable dipeptidase PvdM from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 147.123, 147.123, 147.123 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23300 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.439 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.520 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.172 | 0.359 |
| Number of reflections | 35783 | |
| <I/σ(I)> | 16.4 | 6.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.1 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 100mM Hepes pH 7.0, 3% PEG 3350, 5mM Calcium chloride, 5mM Nickel chloride hexahydrate, 5mM Magnesium chloride hexahydrate, 5mM Cadmium chloride, VAPOR DIFFUSION, temperature 294K |
