3B39
Structure of the DnaG primase catalytic domain bound to ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.111 |
| Spacegroup name | P 62 |
| Unit cell lengths | 136.829, 136.829, 71.071 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.350 |
| R-factor | 0.21398 |
| Rwork | 0.212 |
| R-free | 0.25443 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dde |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.185 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.065 | 0.458 |
| Number of reflections | 30546 | |
| <I/σ(I)> | 14.8 | 2.2 |
| Completeness [%] | 95.7 | 97.3 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 21% PEG 4000, 100 mM ammonium acetate, 50 mM sodium acetate (pH 5.0), VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | ammonium acetate | ||
| 3 | 1 | 1 | sodium acetate | ||
| 4 | 1 | 2 | PEG 4000 | ||
| 5 | 1 | 2 | ammonium acetate | ||
| 6 | 1 | 2 | sodium acetate |
