3AZW

Crystal structure of the receptor binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-28
Detector	ADSC QUANTUM 210r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	57.958, 141.550, 162.039
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.220 - 2.990
R-factor	0.25691
Rwork	0.255
R-free	0.29096
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.026
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.6.0091)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	3.150
High resolution limit [Å]	2.990	2.990
Number of reflections	27614
<I/σ(I)>		2.69
Completeness [%]	99.3	95.6
Redundancy		8.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.1M MES, 1.6M magnesium sulfate, 1M sodium chloride, pH 6.5, VAPOR DIFFUSION, temperature 293K