3AZU

X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	109.740, 99.150, 47.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.100
R-factor	0.163 *
Rwork	0.163
RMSD bond length	0.012
RMSD bond angle	2.870
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.090 *	2.090 *
Rmerge	0.097 *
Total number of observations	99146 *
Number of reflections	22537 *
Completeness [%]	66.6 *	21.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	3.5-3.8 (M)
2	1	reservoir	lithium nitrate	0.5 (M)
3	1	reservoir	acetate	0.1 (M)	can be replaced with MES
4	1	reservoir	protein	12-17 (mg/ml)