3AZC
Crystal structure of the soluble part of cytochrome b6f complex iron-sulfur subunit from Thermosynechococcus elongatus BP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 40 |
| Detector technology | CCD |
| Collection date | 2011-02-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.65 |
| Spacegroup name | P 65 |
| Unit cell lengths | 91.324, 91.324, 43.342 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.610 - 2.000 |
| Rwork | 0.197 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e74 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 24682 | |
| <I/σ(I)> | 32.2 | 2.1 |
| Completeness [%] | 87.4 | 57.1 |
| Redundancy | 7.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Na-HEPES, 10% 2-propanol, 20% PEG 3000, 15% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
