3AYM
Crystal structure of the batho intermediate of squid rhodopsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW14A |
Synchrotron site | Photon Factory |
Beamline | AR-NW14A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 62 |
Unit cell lengths | 122.360, 122.360, 158.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
Rwork | 0.295 |
R-free | 0.34480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z73 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.078 | 0.529 |
Number of reflections | 31468 | |
<I/σ(I)> | 11.2 | 2 |
Completeness [%] | 95.5 | 95 |
Redundancy | 3.3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 277 | 3.2M ammonium sulfate, 20mM MES, 75mM EDTA, 10mM beta-mercaptoethanol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |