3AYE
Crystal structure of galectin-3 CRD domian complexed with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 85 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.640, 51.670, 60.100 |
| Unit cell angles | 64.37, 86.25, 80.47 |
Refinement procedure
| Resolution | 32.190 - 2.000 |
| Rwork | 0.175 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nn8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.190 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.029 | 0.058 |
| Number of reflections | 22109 | |
| <I/σ(I)> | 12.7 | 7.4 |
| Completeness [%] | 93.8 | 91.1 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 2.0 A.S., 0.1M Bis-Tris, 5mM beta-Mercaptoethanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
