3AY5

Crystal structure of HHM (human homologue of murine maternal Id-like molecule)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	105.031, 105.031, 124.266
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.624 - 2.500
R-factor	0.2239
Rwork	0.222
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.029
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.046	0.023	0.371
Number of reflections	25654
<I/σ(I)>	26
Completeness [%]	91.8	74.9	91.6
Redundancy	6.8	8.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	100mM imidazole, 500mM (NH4)2HPO4, 100mM NaCl, pH 8.0, vapor diffusion, sitting drop, temperature 293K