3AXM
Structure of rice Rubisco in complex with 6PG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-23 |
| Detector | OTHERS |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.408, 199.624, 111.169 |
| Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
| Resolution | 74.260 - 1.650 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.260 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.056 | 0.311 |
| Number of reflections | 511171 | |
| Completeness [%] | 89.3 | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 75mM HEPES-KOH(pH 7.75), 9% PEG4000, 25% glycerol, VAPOR DIFFUSION, temperature 293K |
