3AXE
The truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase V18Y/W203Y in complex with cellotetraose (cellobiose density was observed)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 160 |
Detector technology | CCD |
Collection date | 2010-06-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.091, 70.404, 76.589 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.530 |
Rwork | 0.182 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zm1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | XTALVIEW |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.580 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.056 | 0.390 |
Number of reflections | 32609 | |
<I/σ(I)> | 30.7 | 4.2 |
Completeness [%] | 98.1 | 98.1 |
Redundancy | 5.9 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.15M Tris, pH 8.5, 0.4M Calcium acetate, 33% PEG 5KMME, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |