3AWE
Crystal structure of Pten-like domain of Ci-VSP (248-576)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-05 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 131.207, 176.527, 50.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.130 - 2.770 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d5r |
RMSD bond length | 0.019 |
RMSD bond angle | 1.664 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | REFMAC (5.5.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.118 | 0.439 |
Number of reflections | 30351 | |
<I/σ(I)> | 13.383 | |
Completeness [%] | 98.5 | 98.2 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.1M AMMONIUM SULFATE, 0.1M TRIS, 0.5M SODIUM FLUORIDE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |