3AV3
Crystal structure of glycinamide ribonucleotide transformylase 1 from Geobacillus kaustophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-15 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97944, 0.90000, 0.97984 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.861, 117.408, 67.251 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.770 - 1.700 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.21400 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.045 | 0.115 |
| Number of reflections | 43718 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | Bis-Tris 0.1M pH 6.4, 0.5M Sodium Fluoride, PEG 3350 25%, Mg Chloride 0.1M, VAPOR DIFFUSION, SITTING DROP |
