3AUY
Crystal structure of Rad50 bound to ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.160, 95.300, 110.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.400 - 2.700 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ii8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.300 | 2.720 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 24959 | |
| Completeness [%] | 98.8 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 9.5% PEG 8000, 0.1M sodium cacodylate pH6.5, 0.1M magnessium acetate, 1mM ADP, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
