3AUW

Cytoplasmic domain of inward rectifier potassium channel Kir3.2 in complex with cadmium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-06
Detector	RAYONIX MX225HE
Wavelength(s)	1.00000
Spacegroup name	P 4 21 2
Unit cell lengths	100.808, 100.808, 105.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.560
R-factor	0.2865
Rwork	0.281
R-free	0.33110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.763
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.560
Rmerge	0.206
Number of reflections	26865
<I/σ(I)>	6.714
Completeness [%]	99.0
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K