3ATO

Glycine ethyl ester shielding on the aromatic surfaces of lysozyme: Implication for suppression of protein aggregation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32B2
Synchrotron site	SPring-8
Beamline	BL32B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-12-08
Detector	RIGAKU RAXIS V
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	77.171, 77.171, 36.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.570 - 1.170
R-factor	0.155
Rwork	0.154
R-free	0.17480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.584
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.3_473))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.210
High resolution limit [Å]	1.170	1.170
Number of reflections	36120
Completeness [%]	99.4	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch	4.5	293	0.05M sodium acetate, 1.0M glycine ethyl ester, pH 4.5, Batch, temperature 293K