3ASY

ligand-free structure of uridine kinase from thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	70.364, 70.364, 179.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.800 - 2.400
Rwork	0.233
R-free	0.29950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	uridine cytidine kinase2 from Homo sapiens
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge		0.640
Number of reflections	18465
<I/σ(I)>		6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	40% (v/v) isopropanol, 15%(w/v) PEG 8000, 0.1M imidazole, pH 6.5, VAPOR DIFFUSION, temperature 293K