3ASL
Structure of UHRF1 in complex with histone tail
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.28221 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.210, 36.137, 34.175 |
| Unit cell angles | 90.00, 110.69, 90.00 |
Refinement procedure
| Resolution | 31.970 - 1.410 |
| R-factor | 0.16052 |
| Rwork | 0.159 |
| R-free | 0.19621 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.512 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 14019 | |
| Completeness [%] | 92.6 | 79.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10mM sodium citrate, 42% PEGMME2000, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10mM sodium citrate, 42% PEGMME2000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
