3ASG
MamA D159K mutant 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.570, 76.471, 104.587 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.720 - 2.330 |
| R-factor | 0.2067 |
| Rwork | 0.204 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3as5 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.516 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.330 | 6.370 | 2.350 |
| Rmerge | 0.123 | 0.047 | 0.483 |
| Number of reflections | 15590 | ||
| <I/σ(I)> | 8 | ||
| Completeness [%] | 98.6 | 98.5 | 98.7 |
| Redundancy | 5.2 | 5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 286 | MgCl, PEG 3350, Tris, NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
