3ASF
MamA MSR-1 C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.129, 73.377, 90.224 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 38.200 - 2.390 |
| R-factor | 0.2482 |
| Rwork | 0.247 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3as5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.374 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.440 |
| High resolution limit [Å] | 2.390 | 6.510 | 2.400 |
| Rmerge | 0.079 | 0.041 | 0.558 |
| Number of reflections | 22523 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 99.8 | 97.3 | 100 |
| Redundancy | 7.3 | 6.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 286 | Ammonium sulfat, HEPES, NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
