3AS5
MamA AMB-1 P212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.750, 76.194, 105.057 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.830 - 2.000 |
R-factor | 0.1791 |
Rwork | 0.176 |
R-free | 0.24100 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.666 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.410 | 2.000 |
Rmerge | 0.063 | 0.045 | 0.259 |
Number of reflections | 24938 | ||
<I/σ(I)> | 17.9 | ||
Completeness [%] | 99.7 | 99.1 | 99.8 |
Redundancy | 6.7 | 6 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 286 | Tris, MgCl, PEG 3350, NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 286 | Tris, MgCl, PEG 3350, NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |