3AR2
Calcium pump crystal structure with bound AMPPCP and Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-20 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.75 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.995, 75.306, 151.541 |
| Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.24817 |
| Rwork | 0.247 |
| R-free | 0.27243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zbd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.252 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 59761 | |
| <I/σ(I)> | 31.8 | 2.7 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.1 | 283 | pH 6.1, MICRODIALYSIS, temperature 283K |
