3AQ3
Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.458, 80.458, 245.708 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.920 - 2.100 |
| R-factor | 0.20392 |
| Rwork | 0.202 |
| R-free | 0.23708 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.010 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 43896 | |
| Completeness [%] | 84.0 | 60 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | magnesium formate, Bis-tris, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
