3APZ

Apo form of Arabidopsis medium/long-chain length prenyl pyrophosphate synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-27
Detector	ADSC QUANTUM 4r
Wavelength(s)	1
Spacegroup name	F 2 2 2
Unit cell lengths	148.512, 150.126, 176.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
Rwork	0.206
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wy0
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.071	0.489
Number of reflections	33211
<I/σ(I)>	18.3	2.5
Completeness [%]	99.5	98.8
Redundancy	3.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	295	60%(v/v) Tacsimate, pH 7.0, vapor diffusion, temperature 295K