3APW
Crystal structure of the A variant of human alpha1-acid glycoprotein and disopyramide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.720, 45.490, 120.600 |
| Unit cell angles | 90.00, 91.69, 90.00 |
Refinement procedure
| Resolution | 37.760 - 2.200 |
| R-factor | 0.23005 |
| Rwork | 0.228 |
| R-free | 0.26339 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3apu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.291 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.190 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.058 | 0.105 |
| Number of reflections | 18846 | |
| <I/σ(I)> | 26.2 | 15.2 |
| Completeness [%] | 99.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 30% PEG 4000, 0.2M ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
