3APB

Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with iodide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-03
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.30000
Spacegroup name	P 21 21 21
Unit cell lengths	65.591, 75.869, 77.901
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.950 - 1.950
R-factor	0.20342
Rwork	0.202
R-free	0.24198
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.318
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.954	2.010
High resolution limit [Å]	1.947	1.940
Number of reflections	28969
Completeness [%]	99.9	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	0.55mM protein, 10mM Hepes-NaOH, 50mM sodium chloride, 0.5mM DTT, 125mM ammonium fluoride, 8% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K