3APB
Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with iodide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-03 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.30000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.591, 75.869, 77.901 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.950 - 1.950 |
| R-factor | 0.20342 |
| Rwork | 0.202 |
| R-free | 0.24198 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.318 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.954 | 2.010 |
| High resolution limit [Å] | 1.947 | 1.940 |
| Number of reflections | 28969 | |
| Completeness [%] | 99.9 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.55mM protein, 10mM Hepes-NaOH, 50mM sodium chloride, 0.5mM DTT, 125mM ammonium fluoride, 8% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
