3AP6
Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose 3'-sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-10-20 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.977980 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.376, 65.400, 71.693 |
| Unit cell angles | 98.29, 105.43, 108.55 |
Refinement procedure
| Resolution | 21.440 - 1.580 |
| R-factor | 0.2018 |
| Rwork | 0.200 |
| R-free | 0.24179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ap5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.423 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.507 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.055 | 0.121 |
| Number of reflections | 86082 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 94.4 | 91.5 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose 3'-sulfate, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 9% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
