3AP4
Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-03 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.92000 |
Spacegroup name | P 1 |
Unit cell lengths | 41.528, 64.888, 71.584 |
Unit cell angles | 98.41, 105.49, 108.66 |
Refinement procedure
Resolution | 51.330 - 2.330 |
R-factor | 0.19364 |
Rwork | 0.191 |
R-free | 0.25138 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.205 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.462 | 2.390 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.046 | 0.060 |
Number of reflections | 26924 | |
<I/σ(I)> | 10.7 | |
Completeness [%] | 95.5 | 76.3 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 12% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |