3AP3
Crystal structure of human tyrosylprotein sulfotransferase-2 complexed with PAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 71.432, 81.326, 226.659 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.500 - 3.500 |
R-factor | 0.29398 |
Rwork | 0.290 |
R-free | 0.37090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ap1 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.329 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.164 | 0.464 |
Number of reflections | 11529 | |
<I/σ(I)> | 16.5 | 3 |
Completeness [%] | 68.1 | 42.9 |
Redundancy | 7.7 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283.4 | 0.1M Tris-HCl, pH 7.0, 0.1M calcium acetate, 20 % polyethylene glycol 3000, VAPOR DIFFUSION, SITTING DROP, temperature 283.4K |