3AP1
Crystal structure of human tyrosylprotein sulfotransferase-2 complexed with PAP and C4 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 138.263, 138.263, 227.994 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.300 - 1.900 |
| R-factor | 0.19658 |
| Rwork | 0.195 |
| R-free | 0.22165 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.455 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.108 | 0.108 |
| Number of reflections | 65960 | |
| <I/σ(I)> | 12.5 | 12.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.4 | 0.1 M sodium malonate (pH 5.0), 10% polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293.4K |
