3AO9

Crystal structure of the C-terminal domain of sequence-specific ribonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-09
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	62.710, 62.710, 100.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.780 - 2.100
R-factor	0.22359
Rwork	0.222
R-free	0.25866
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2djh
RMSD bond length	0.022
RMSD bond angle	1.957
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	13723
Completeness [%]	98.6	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	278	50mM MES, 0.1M cadmium chloride, 25% PEG MME, 15% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K