3AMY
Crystal structure of human CK2 alpha complexed with apigenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.630, 73.030, 77.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.220 - 2.300 |
| R-factor | 0.26896 |
| Rwork | 0.267 |
| R-free | 0.34029 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zjw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.180 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Total number of observations | 85536 | |
| Number of reflections | 12614 | |
| Completeness [%] | 98.8 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 25% ethylene glycol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
