3AM1
Crystal structure of O-Phosphoseryl-tRNA kinase complexed with anticodon-stem/loop truncated tRNA(Sec)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 46.749, 46.749, 459.712 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.463 - 2.400 |
| R-factor | 0.2324 |
| Rwork | 0.229 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a4n |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.536 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.111 | 0.054 | 0.392 |
| Number of reflections | 22946 | ||
| <I/σ(I)> | 25.886 | ||
| Completeness [%] | 97.6 | 97.6 | 79.4 |
| Redundancy | 14.3 | 19.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 100mM phosphate buffer (pH 6.2), 0.2M NaCl, 41% PEG 200, vapor diffusion, sitting drop, temperature 293K, VAPOR DIFFUSION, SITTING DROP |
